5irf

X-ray diffraction
1.6Å resolution

Reverse transcriptase domain of group II intron maturase from Roseburia intestinalis in P1 space group

Released:
Source organism: Roseburia intestinalis XB6B4
Primary publication:
Crystal structures of a group II intron maturase reveal a missing link in spliceosome evolution.
OpenAccess logo Nat Struct Mol Biol 23 558-65 (2016)
PMID: 27136328

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Reverse transcriptase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 305 amino acids
Theoretical weight: 34.92 KDa
Source organism: Roseburia intestinalis XB6B4
Expression system: Escherichia coli
UniProt:
  • Canonical: D4L313 (Residues: 1-305; Coverage: 70%)
Gene name: RO1_37670
Sequence domains: Reverse transcriptase (RNA-dependent DNA polymerase)

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P1
Unit cell:
a: 50.29Å b: 79.16Å c: 86.02Å
α: 109° β: 90.56° γ: 105.04°
R-values:
R R work R free
0.192 0.191 0.227
Expression system: Escherichia coli