› 5irf

X-ray diffraction
1.6Å resolution

Reverse transcriptase domain of group II intron maturase from Roseburia intestinalis in P1 space group

Released:
DOI: 10.2210/pdb5irf/pdb
PDB 5irf coloured by chain and viewed from the front.
PDB 5irf coloured by chain and viewed from the side.
PDB 5irf coloured by chain and viewed from the top.
Source organism: Roseburia intestinalis XB6B4
Primary publication:
Crystal structures of a group II intron maturase reveal a missing link in spliceosome evolution.
Zhao C, Pyle AM
OpenAccess logo Nat Struct Mol Biol 23 558-65 (2016)
PMID: 27136328

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Reverse transcriptase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 305 amino acids
Theoretical weight: 34.92 KDa
Source organism: Roseburia intestinalis XB6B4
Expression system: Escherichia coli
UniProt:
  • Canonical: D4L313 (Residues: 1-305; Coverage: 70%)
Gene name: RO1_37670
Sequence domains: Reverse transcriptase (RNA-dependent DNA polymerase)

Ligands and Environments

1 bound ligand:
Ligand K 4 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P1
Unit cell:
a: 50.29Å b: 79.16Å c: 86.02Å
α: 109° β: 90.56° γ: 105.04°
R-values:
R R work R free
0.192 0.191 0.227
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Prokaryotic reverse transcriptases: from retroelements to specialized defense systems.
González-Delgado et al. (2021)
14 more