› 5ir3

X-ray diffraction
1.7Å resolution

Crystal structure of the recombinant highest fibrillogenic natural mutant (obtained from patient AR) derived from lambda 6 light chain variable domain

Released:
DOI: 10.2210/pdb5ir3/pdb
PDB entry 5ir3 coloured by chain, front view.
PDB entry 5ir3 coloured by chain, side view.
PDB entry 5ir3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Stabilizing an amyloidogenic λ6 light chain variable domain.
Luna-Martínez OD, Hernández-Santoyo A, Villalba-Velázquez MI, Sánchez-Alcalá R, Fernández-Velasco DA, Becerril B
FEBS J 284 3702-3717 (2017)
PMID: 28898537

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134495 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin lambda variable 6-57 Chain: A
Molecule details ›
Chain: A
Length: 112 amino acids
Theoretical weight: 11.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01721 (Residues: 20-117; Coverage: 100%)
Gene name: IGLV6-57
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: I4122
Unit cell:
a: 71.121Å b: 71.121Å c: 96.453Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.207
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Genetic pathogenesis of immunoglobulin light chain amyloidosis: basic characteristics and clinical applications.
Xu et al. (2021)
2 other articles

3 mentions without citation

Role of the mechanisms for antibody repertoire diversification in monoclonal light chain deposition disorders: when a friend becomes foe.
Del Pozo-Yauner et al. (2023)
2 more