› 5iqs

X-ray diffraction
2Å resolution

WelO5 bound to Fe(II), Cl, and 2-oxoglutarate

Released:
DOI: 10.2210/pdb5iqs/pdb
PDB entry 5iqs coloured by chain, front view.
PDB entry 5iqs coloured by chain, side view.
PDB entry 5iqs coloured by chain, top view.
Source organism: Hapalosiphon welwitschii UTEX B 1830
Primary publication:
Structural basis for halogenation by iron- and 2-oxo-glutarate-dependent enzyme WelO5.
Mitchell AJ, Zhu Q, Maggiolo AO, Ananth NR, Hillwig ML, Liu X, Boal AK
Nat Chem Biol 12 636-40 (2016)
PMID: 27348090

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100382 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
WelO5 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 315 amino acids
Theoretical weight: 35.53 KDa
Source organism: Hapalosiphon welwitschii UTEX B 1830
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A067YX61 (Residues: 1-290; Coverage: 100%)

Ligands and Environments

3 bound ligands:
Ligand FE2 3 x FE2
Ligand AKG 3 x AKG
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 70.757Å b: 86.339Å c: 141.312Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.219
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Oxygen Activation and Radical Transformations in Heme Proteins and Metalloporphyrins.
Huang et al. (2018)
16 more

4 mentions without citation

Recent examples of α-ketoglutarate-dependent mononuclear non-haem iron enzymes in natural product biosyntheses.
Gao et al. (2018)
3 more