5inb

X-ray diffraction
1.3Å resolution

RepoMan-PP1g (protein phosphatase 1, gamma isoform) holoenzyme complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein phosphatase PP1-gamma catalytic subunit Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 35.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P36873 (Residues: 7-308; Coverage: 94%)
Gene name: PPP1CC
Sequence domains:
Structure domains: Purple Acid Phosphatase; chain A, domain 2
Cell division cycle-associated protein 2 Chain: B
Molecule details ›
Chain: B
Length: 46 amino acids
Theoretical weight: 5.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q69YH5 (Residues: 383-423; Coverage: 4%)
Gene name: CDCA2

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P6122
Unit cell:
a: 86.371Å b: 86.371Å c: 215.894Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.133 0.132 0.153
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli