X-ray diffraction
2.1Å resolution

Tobacco 5-epi-aristolochene synthase with CAPSO buffer molecule and Ca2+ ions

Source organism: Nicotiana tabacum
Entry authors: Koo HJ, Louie GV, Xu Y, Bowman M, Noel JP

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
5-epi-aristolochene synthase Chain: A
Molecule details ›
Chain: A
Length: 550 amino acids
Theoretical weight: 63.2 KDa
Source organism: Nicotiana tabacum
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q40577 (Residues: 1-548; Coverage: 100%)
Gene names: EAS3, EAS4
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P41212
Unit cell:
a: 126.28Å b: 126.28Å c: 123.27Å
α: 90° β: 90° γ: 90°
R R work R free
0.186 0.185 0.217
Expression system: Escherichia coli BL21(DE3)