5ikn

X-ray diffraction
4.8Å resolution

Crystal Structure of the T7 Replisome in the Absence of DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
DNA-directed DNA polymerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 704 amino acids
Theoretical weight: 79.7 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P00581 (Residues: 1-704; Coverage: 100%)
Gene name: 5
Sequence domains: DNA polymerase family A
DNA helicase/primase Chains: D, E, F, G, H, I, J
Molecule details ›
Chains: D, E, F, G, H, I, J
Length: 486 amino acids
Theoretical weight: 53.82 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P03692 (Residues: 64-549; Coverage: 86%)
Gene name: 4
Sequence domains: DnaB-like helicase C terminal domain
Thioredoxin 1 Chains: K, L, M
Molecule details ›
Chains: K, L, M
Length: 105 amino acids
Theoretical weight: 11.41 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA27 (Residues: 4-108; Coverage: 96%)
Gene names: ECs4714, Z5291, trxA
Sequence domains: Thioredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 174.866Å b: 238.091Å c: 243.466Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.277 0.275 0.318
Expression system: Escherichia coli