› 5ij6

X-ray diffraction
2Å resolution

Crystal structure of Enterococcus faecalis lipoate-protein ligase A (lplA-1) in complex with lipoic acid

Released:
DOI: 10.2210/pdb5ij6/pdb
PDB 5ij6 coloured by chain and viewed from the front.
PDB 5ij6 coloured by chain and viewed from the side.
PDB 5ij6 coloured by chain and viewed from the top.
Source organism: Enterococcus faecalis V583
Entry authors: Hughes SJ, Lyle AG, Song JH, Antoshchenko T, Park H

Function and Biology Details

Reaction catalysed: 
(1a) ATP + (R)-lipoate = lipoyl-AMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
lipoate--protein ligase Chain: A
Molecule details ›
Chain: A
Length: 355 amino acids
Theoretical weight: 40.78 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q838C1 (Residues: 1-337; Coverage: 100%)
Gene names: EF_0650, lplA-1
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand LPA 1 x LPA
Ligand SO4 1 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 53.011Å b: 70.912Å c: 106.466Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.178 0.207
Expression system: Escherichia coli BL21(DE3)

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