X-ray diffraction
1.55Å resolution

Macrolide 2'-phosphotransferase type II - complex with GDP and azithromycin

Source organism: Escherichia coli
Primary publication:
Structural Basis for Kinase-Mediated Macrolide Antibiotic Resistance.
Structure 25 750-761.e5 (2017)
PMID: 28416110

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
APH domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
  • Canonical: O32553 (Residues: 1-302; Coverage: 100%)
Gene names: BON70_03915, DAH50_23490, GF147_25890, GF147_27035, HVY77_26855, mph(B), mphB, pO103_99
Sequence domains: Phosphotransferase enzyme family

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 40.23Å b: 80.45Å c: 96.43Å
α: 90° β: 90° γ: 90°
R R work R free
0.179 0.178 0.209
Expression system: Escherichia coli BL21(DE3)