› 5igv

X-ray diffraction
1.55Å resolution

Macrolide 2'-phosphotransferase type II - complex with GDP and azithromycin

Released:
DOI: 10.2210/pdb5igv/pdb
PDB 5igv coloured by chain and viewed from the front.
PDB 5igv coloured by chain and viewed from the side.
PDB 5igv coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Structural Basis for Kinase-Mediated Macrolide Antibiotic Resistance.
Fong DH, Burk DL, Blanchet J, Yan AY, Berghuis AM
Structure 25 750-761.e5 (2017)
PMID: 28416110

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
APH domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O32553 (Residues: 1-302; Coverage: 100%)
Gene names: BON70_03915, DAH50_23490, GF147_25890, GF147_27035, HVY77_26855, mph(B), mphB, pO103_99
Sequence domains: Phosphotransferase enzyme family

Ligands and Environments

4 bound ligands:
Ligand MG 1 x MG
Ligand CA 1 x CA
Ligand GDP 1 x GDP
Ligand ZIT 1 x ZIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 40.23Å b: 80.45Å c: 96.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.178 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Comparison of Antibiotic Resistance Mechanisms in Antibiotic-Producing and Pathogenic Bacteria.
Ogawara H. (2019)
3 more