5idt

X-ray diffraction
2.35Å resolution

Crystal Structure of a Glucose-1-phosphate Thymidylyltransferase from Burkholderia vietnamiensis with bound Thymidine

Released:
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed:
dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-1-phosphate thymidylyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 302 amino acids
Theoretical weight: 33.32 KDa
Source organism: Burkholderia vietnamiensis G4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4JIV2 (Residues: 1-294; Coverage: 100%)
Gene name: Bcep1808_3215
Sequence domains: Nucleotidyl transferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2
Unit cell:
a: 112.11Å b: 114.86Å c: 95.78Å
α: 90° β: 90.58° γ: 90°
R-values:
R R work R free
0.178 0.176 0.231
Expression system: Escherichia coli BL21(DE3)