› 5iby

X-ray diffraction
1.85Å resolution

Crystal structure of Enterococcus faecalis lipoate-protein ligase A (lplA-2) in complex with lipoic acid

Released:
DOI: 10.2210/pdb5iby/pdb
PDB entry 5iby coloured by chain, front view.
PDB entry 5iby coloured by chain, side view.
PDB entry 5iby coloured by chain, top view.
Source organism: Enterococcus faecalis V583
Entry authors: Hughes SJ, Song JH, Antoshchenko T, Park HW

Function and Biology Details

Reaction catalysed: 
(1a) ATP + (R)-lipoate = lipoyl-AMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182756 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
lipoate--protein ligase Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 40.55 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q830N7 (Residues: 1-334; Coverage: 100%)
Gene names: EF_2741, lplA-2
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand LPA 1 x LPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 50.977Å b: 68.561Å c: 54.154Å
α: 90° β: 116.13° γ: 90°
R-values:
R R work R free
0.166 0.164 0.21
Expression system: Escherichia coli BL21(DE3)

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