› 5ibw

X-ray diffraction
1.9Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154658 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
EF-hand domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 77 amino acids
Theoretical weight: 8.92 KDa
Source organism: Dictyostelium discoideum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q54HC2 (Residues: 1-74; Coverage: 100%)
Gene name: DDB_G0289563
Sequence domains: EF-hand domain pair
Structure domains: EF-hand
Myosin IC heavy chain Chain: C
Molecule details ›
Chain: C
Length: 43 amino acids
Theoretical weight: 5.46 KDa
Source organism: Dictyostelium discoideum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42522 (Residues: 699-739; Coverage: 4%)
Gene names: DDB_G0276617, dmiC, myoC

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P1
Unit cell:
a: 28.68Å b: 31.41Å c: 56.41Å
α: 75.79° β: 84° γ: 67.06°
R-values:
R R work R free
0.14 0.14 0.19
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Calmodulin and Calmodulin Binding Proteins in Dictyostelium: A Primer.
O'Day et al. (2020)
3 more

1 mention without citation

Structure of the Single-lobe Myosin Light Chain C in Complex with the Light Chain-binding Domains of Myosin-1C Provides Insights into Divergent IQ Motif Recognition.
Langelaan et al. (2016)