X-ray diffraction
2.19Å resolution

Crystal structure of designed pentatricopeptide repeat protein dPPR-U10 in complex with its target RNA U10


Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
pentatricopeptide repeat protein dPPR-U10 Chain: A
Molecule details ›
Chain: A
Length: 460 amino acids
Theoretical weight: 51.52 KDa
Source organism: unidentified
Expression system: Escherichia coli BL21(DE3)
Molecule details ›
Chain: F
Length: 18 nucleotides
Theoretical weight: 5.62 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 52.732Å b: 85.266Å c: 95.104Å
α: 90° β: 90° γ: 90°
R R work R free
0.253 0.25 0.299
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided