› 5i6c

X-ray diffraction
3.7Å resolution

The structure of the eukaryotic purine/H+ symporter, UapA, in complex with Xanthine

Released:
DOI: 10.2210/pdb5i6c/pdb
PDB 5i6c coloured by chain and viewed from the front.
PDB 5i6c coloured by chain and viewed from the side.
PDB 5i6c coloured by chain and viewed from the top.
Source organism: Aspergillus nidulans FGSC A4
Primary publication:
Structure of eukaryotic purine/H(+) symporter UapA suggests a role for homodimerization in transport activity.
Alguel Y, Amillis S, Leung J, Lambrinidis G, Capaldi S, Scull NJ, Craven G, Iwata S, Armstrong A, Mikros E, Diallinas G, Cameron AD, Byrne B
Nat Commun 7 11336 (2016)
PMID: 27088252

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uric acid-xanthine permease Chains: A, B
Molecule details ›
Chains: A, B
Length: 574 amino acids
Theoretical weight: 61.2 KDa
Source organism: Aspergillus nidulans FGSC A4
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q07307 (Residues: 1-574; Coverage: 100%)
Gene names: AN6932, uapA
Sequence domains: Permease family

Ligands and Environments

2 bound ligands:
Ligand XAN 2 x XAN
Ligand LMT 4 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 75.09Å b: 173.83Å c: 82.39Å
α: 90° β: 111.11° γ: 90°
R-values:
R R work R free
0.298 0.296 0.327
Expression system: Saccharomyces cerevisiae

Quick links

Citations

7 review citations

Organization and Dynamics of the Red Blood Cell Band 3 Anion Exchanger SLC4A1: Insights From Molecular Dynamics Simulations.
Kalli et al. (2022)
6 more

10 mentions without citation

Assessing the Role of Lipids in the Molecular Mechanism of Membrane Proteins.
Jodaitis et al. (2021)
9 more