› 5i62

X-ray diffraction
2Å resolution

Crystal structure of the insertion loop deletion mutant of the RNA-dependent RNA polymerase of a human picorbirnavirus

Released:
DOI: 10.2210/pdb5i62/pdb
PDB entry 5i62 coloured by chain, front view.
PDB entry 5i62 coloured by chain, side view.
PDB entry 5i62 coloured by chain, top view.
Source organism: Human picobirnavirus strain Hy005102
Primary publication:
Initiation of RNA Polymerization and Polymerase Encapsidation by a Small dsRNA Virus.
Collier AM, Lyytinen OL, Guo YR, Toh Y, Poranen MM, Tao YJ
PLoS Pathog 12 e1005523 (2016)
PMID: 27078841

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175901 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potential RNA-dependent RNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 534 amino acids
Theoretical weight: 60.98 KDa
Source organism: Human picobirnavirus strain Hy005102
Expression system: Escherichia coli
UniProt:
  • Canonical: Q50LE4 (Residues: 2-534; Coverage: 100%)
Gene names: ORF3, Segment-2
Sequence domains: Viral RNA-dependent RNA polymerase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P41212
Unit cell:
a: 77.534Å b: 77.534Å c: 183.858Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.201
Expression system: Escherichia coli

Quick links

Citations

6 review citations

RNA Dependent RNA Polymerases: Insights from Structure, Function and Evolution.
Venkataraman et al. (2018)
5 more

2 mentions without citation

Initiation of RNA Polymerization and Polymerase Encapsidation by a Small dsRNA Virus.
Collier et al. (2016)
1 more