5i4r

X-ray diffraction
3.3Å resolution

Contact-dependent inhibition system from Escherichia coli NC101 - ternary CdiA/CdiI/EF-Tu complex (trypsin-modified)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-143214 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
tRNA nuclease CdiA Chains: A, E
Molecule details ›
Chains: A, E
Length: 92 amino acids
Theoretical weight: 10.48 KDa
Source organism: Escherichia coli NC101
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DSI1 (Residues: 3198-3289; Coverage: 3%)
Gene names: ECNC101_09164, cdiA
Elongation factor Tu 2 Chains: C, G
Molecule details ›
Chains: C, G
Length: 45 amino acids
Theoretical weight: 4.8 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CE48 (Residues: 1-45; Coverage: 11%)
Gene names: JW3943, b3980, tufB
Elongation factor Tu 1 Chains: D, H
Molecule details ›
Chains: D, H
Length: 335 amino acids
Theoretical weight: 36.97 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CE47 (Residues: 60-394; Coverage: 85%)
Gene names: JW3301, b3339, tufA
Sequence domains:
Structure domains:
Immunity protein CdiI Chains: B, F
Molecule details ›
Chains: B, F
Length: 114 amino acids
Theoretical weight: 14.23 KDa
Source organism: Escherichia coli NC101
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DSM8 (Residues: 1-106; Coverage: 100%)
Gene names: ECNC101_09169, cdiI

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 74.395Å b: 128.354Å c: 100.358Å
α: 90° β: 109.58° γ: 90°
R-values:
R R work R free
0.244 0.243 0.268
Expression system: Escherichia coli BL21(DE3)