› 5i30

X-ray diffraction
1.9Å resolution

Crystal structure of the human astrovirus 2 neutralizing monoclonal antibody PL-2 Fab fragment at 1.9 A resolution

Released:
DOI: 10.2210/pdb5i30/pdb
PDB 5i30 coloured by chain and viewed from the front.
PDB 5i30 coloured by chain and viewed from the side.
PDB 5i30 coloured by chain and viewed from the top.
Source organism: Mus musculus
Primary publication:
De Novo Sequencing and Resurrection of a Human Astrovirus-Neutralizing Antibody.
Bogdanoff WA, Morgenstern D, Bern M, Ueberheide BM, Sanchez-Fauquier A, DuBois RM
ACS Infect Dis 2 313-321 (2016)
PMID: 27213181

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fab PL-2 light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.68 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Fab PL-2 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 221 amino acids
Theoretical weight: 23.57 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: C2221
Unit cell:
a: 72.22Å b: 170.47Å c: 78.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.221

Quick links

Citations

3 review citations

Structural Insights into the Human Astrovirus Capsid.
Ykema et al. (2021)
2 more

3 mentions without citation

De Novo Sequencing and Resurrection of a Human Astrovirus-Neutralizing Antibody.
Bogdanoff et al. (2016)
2 more