› 5i2h

X-ray diffraction
1.55Å resolution

Crystal structure of O-methyltransferase family 2 protein Plim_1147 from Planctomyces limnophilus DSM 3776 complex with Apigenin

Released:
DOI: 10.2210/pdb5i2h/pdb
PDB entry 5i2h coloured by chain, front view.
PDB entry 5i2h coloured by chain, side view.
PDB entry 5i2h coloured by chain, top view.
Source organism: Planctopirus limnophila DSM 3776
Entry authors: Chang C, Duke N, Bigelow L, Bearden J, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112737 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-methyltransferase family 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 364 amino acids
Theoretical weight: 39.77 KDa
Source organism: Planctopirus limnophila DSM 3776
Expression system: Escherichia coli
UniProt:
  • Canonical: D5STZ7 (Residues: 1-361; Coverage: 100%)
Gene name: Plim_1147
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand AGI 2 x AGI
Ligand EDO 10 x EDO
Ligand FMT 8 x FMT
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 66.516Å b: 83.192Å c: 119.099Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.197
Expression system: Escherichia coli

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