› 5i21

X-ray diffraction
1.55Å resolution

Y55W Hfq from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb5i21/pdb
PDB 5i21 coloured by chain and viewed from the front.
PDB 5i21 coloured by chain and viewed from the side.
PDB 5i21 coloured by chain and viewed from the top.
Source organism: Pseudomonas aeruginosa PA7
Entry authors: Nikulin AD, Lekontseva NV

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-binding protein Hfq Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 82 amino acids
Theoretical weight: 9.14 KDa
Source organism: Pseudomonas aeruginosa PA7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A6VD57 (Residues: 1-82; Coverage: 100%)
Gene names: PSPA7_5673, hfq
Sequence domains: Hfq protein
Structure domains: SH3 type barrels.

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P6
Unit cell:
a: 114.362Å b: 114.362Å c: 28.343Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.188 0.214
Expression system: Escherichia coli BL21(DE3)

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