› 5i1u

X-ray diffraction
1.5Å resolution

Crystal structure of germacradien-4-ol synthase from Streptomyces citricolor

Released:
DOI: 10.2210/pdb5i1u/pdb
PDB 5i1u coloured by chain and viewed from the front.
PDB 5i1u coloured by chain and viewed from the side.
PDB 5i1u coloured by chain and viewed from the top.
Source organism: Streptomyces citricolor
Primary publication:
Mechanism of Germacradien-4-ol Synthase-Controlled Water Capture.
Grundy DJ, Chen M, Gonz��lez V, Leoni S, Miller DJ, Christianson DW, Allemann RK
Biochemistry (2016)
PMID: 26998816

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Terpene synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 335 amino acids
Theoretical weight: 38.18 KDa
Source organism: Streptomyces citricolor
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G5EKM9 (Residues: 1-316; Coverage: 100%)
Gene name: SC1
Sequence domains: Terpene synthase family 2, C-terminal metal binding
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P21
Unit cell:
a: 51.369Å b: 74.127Å c: 82.489Å
α: 90° β: 91.72° γ: 90°
R-values:
R R work R free
0.158 0.157 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Structural and Chemical Biology of Terpenoid Cyclases.
Christianson DW. (2017)
2 more

2 mentions without citation

Mechanism of Germacradien-4-ol Synthase-Controlled Water Capture.
Grundy et al. (2016)
1 more