5i0i

X-ray diffraction
3.15Å resolution

Crystal structure of myosin X motor domain with 2IQ motifs in pre-powerstroke state

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Unconventional myosin-X Chains: A, B
Molecule details ›
Chains: A, B
Length: 791 amino acids
Theoretical weight: 91.55 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: Q9HD67 (Residues: 3-793; Coverage: 38%)
Gene names: KIAA0799, MYO10
Sequence domains:
Calmodulin-1 Chain: C
Molecule details ›
Chain: C
Length: 145 amino acids
Theoretical weight: 16.45 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P0DP23 (Residues: 3-147; Coverage: 97%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
Calmodulin-1 Chain: E
Molecule details ›
Chain: E
Length: 145 amino acids
Theoretical weight: 16.37 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P0DP23 (Residues: 3-147; Coverage: 97%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
Calmodulin-1 Chain: G
Molecule details ›
Chain: G
Length: 43 amino acids
Theoretical weight: 4.98 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P0DP23 (Residues: 84-126; Coverage: 29%)
Gene names: CALM, CALM1, CAM, CAM1
Calmodulin-1 Chain: I
Molecule details ›
Chain: I
Length: 64 amino acids
Theoretical weight: 7.41 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P0DP23 (Residues: 84-147; Coverage: 43%)
Gene names: CALM, CALM1, CAM, CAM1

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 113.95Å b: 173.41Å c: 178.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.192 0.218
Expression system: unidentified baculovirus