PDBe 5hzk

X-ray diffraction
3.3Å resolution

Crystal structure of photoinhibitable Intersectin1 containing wildtype LOV2 domain in complex with Cdc42


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cell division control protein 42 homolog Chains: A, C
Molecule details ›
Chains: A, C
Length: 190 amino acids
Theoretical weight: 21.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P60953 (Residues: 1-188; Coverage: 98%)
  • Best match: P60953-1 (Residues: 1-188)
Gene name: CDC42
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Intersectin-1; NPH1-1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 502 amino acids
Theoretical weight: 58.28 KDa
Source organisms: Expression system: Escherichia coli
  • Canonical: Q15811 (Residues: 1230-1308, 1309-1580; Coverage: 20%)
  • Canonical: O49003 (Residues: 404-546; Coverage: 16%)
Gene names: ITSN, ITSN1, NPH1-1, SH3D1A
Sequence domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 112.37Å b: 119.08Å c: 131.08Å
α: 90° β: 90° γ: 90°
R R work R free
0.209 0.207 0.257
Expression system: Escherichia coli