› 5hza

X-ray diffraction
1.35Å resolution

Crystal structure of GII.10 P domain in complex with 3-fucosyllactose (3 FL)

Released:
DOI: 10.2210/pdb5hza/pdb
PDB 5hza coloured by chain and viewed from the front.
PDB 5hza coloured by chain and viewed from the side.
PDB 5hza coloured by chain and viewed from the top.
Source organism: Norovirus GII.10
Primary publication:
Structural Basis for Norovirus Inhibition by Human Milk Oligosaccharides.
Weichert S, Koromyslova A, Singh BK, Hansman S, Jennewein S, Schroten H, Hansman GS
J. Virol. (2016)
PMID: 26889023

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Capsid protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 34.51 KDa
Source organism: Norovirus GII.10
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5F4T5 (Residues: 224-538; Coverage: 58%)
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, FUC, GAL
Ligand BGC 1 x BGC
Ligand FUC 1 x FUC
Ligand GAL 1 x GAL
1 bound ligand:
Ligand EDO 11 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P21
Unit cell:
a: 65.71Å b: 78.17Å c: 71.84Å
α: 90° β: 102.3° γ: 90°
R-values:
R R work R free
0.162 0.161 0.179
Expression system: Escherichia coli BL21

Quick links

Citations

15 review citations

The Antigenic Topology of Norovirus as Defined by B and T Cell Epitope Mapping: Implications for Universal Vaccines and Therapeutics.
van Loben Sels et al. (2019)
14 more