› 5hyj

X-ray diffraction
3.06Å resolution

1E6 TCR in Complex with HLA-A02 carrying AQWGPDPAAA

Released:
DOI: 10.2210/pdb5hyj/pdb
PDB entry 5hyj coloured by chain, front view.
PDB entry 5hyj coloured by chain, side view.
PDB entry 5hyj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A "hotspot" for autoimmune T cells in type 1 diabetes.
Stadinski BD, Obst R, Huseby ES
J Clin Invest 126 2040-2 (2016)
PMID: 27183386

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100922 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chains: A, F
Molecule details ›
Chains: A, F
Length: 276 amino acids
Theoretical weight: 31.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, G
Molecule details ›
Chains: B, G
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Insulin Chains: C, H
Molecule details ›
Chains: C, H
Length: 10 amino acids
Theoretical weight: 983 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 15-24; Coverage: 0%)
Gene name: INS
T cell receptor alpha chain constant; T cell receptor alpha variable 12-3 Chains: D, I
Molecule details ›
Chains: D, I
Length: 193 amino acids
Theoretical weight: 21.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0B4J271 (Residues: 24-113; Coverage: 97%)
  • Canonical: P01848 (Residues: 1-82; Coverage: 59%)
Gene names: TCRA, TRAC, TRAV12-3
Sequence domains:
Structure domains: Immunoglobulins
T cell receptor beta constant 2; T cell receptor beta variable 12-4 Chains: E, J
Molecule details ›
Chains: E, J
Length: 246 amino acids
Theoretical weight: 28.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0B4J2E0 (Residues: 20-115; Coverage: 100%)
  • Canonical: A0A5B9 (Residues: 1-129; Coverage: 73%)
Gene names: TCRBC2, TRBC2, TRBV12-4
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P1
Unit cell:
a: 41.44Å b: 97.31Å c: 121.37Å
α: 97.29° β: 97.66° γ: 92.73°
R-values:
R R work R free
0.216 0.212 0.294
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 citations in other articles

Specificity of bispecific T cell receptors and antibodies targeting peptide-HLA.
Holland et al. (2020)

1 mention without citation

Hotspot autoimmune T cell receptor binding underlies pathogen and insulin peptide cross-reactivity.
Cole et al. (2016)