X-ray diffraction
2Å resolution

Crystal structure of an UDP-forming alpha, alpha-terhalose-phosphate synthase from Burkholderia xenovorans

Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed:
UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Trehalose-6-phosphate synthase Chain: A
Molecule details ›
Chain: A
Length: 483 amino acids
Theoretical weight: 54.39 KDa
Source organism: Paraburkholderia xenovorans LB400
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q142X8 (Residues: 1-475; Coverage: 100%)
Gene name: Bxe_A3374
Sequence domains: Glycosyltransferase family 20
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P6422
Unit cell:
a: 171.18Å b: 171.18Å c: 95.15Å
α: 90° β: 90° γ: 120°
R R work R free
0.158 0.157 0.186
Expression system: Escherichia coli BL21(DE3)