5hw3

X-ray diffraction
1.45Å resolution

Crystal structure of a beta lactamase from Burkholderia vietnamiensis

Released:
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed:
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 269 amino acids
Theoretical weight: 28.91 KDa
Source organism: Burkholderia vietnamiensis G4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4JN42 (Residues: 28-291; Coverage: 100%)
Gene name: Bcep1808_4738
Sequence domains: Beta-lactamase enzyme family
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 41.43Å b: 69.42Å c: 78.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.131 0.129 0.162
Expression system: Escherichia coli BL21(DE3)