› 5hv8

Solution NMR

Solution structure of an octanoyl- loaded acyl carrier protein domain from module MLSA2 of the mycolactone polyketide synthase.

Released:
DOI: 10.2210/pdb5hv8/pdb
PDB entry 5hv8 coloured by chain, ensemble of 20 models, front view.
PDB entry 5hv8 coloured by chain, ensemble of 20 models, side view.
PDB entry 5hv8 coloured by chain, ensemble of 20 models, top view.
Source organism: Mycobacterium ulcerans
Primary publication:
Sticky swinging arm dynamics: studies of an acyl carrier protein domain from the mycolactone polyketide synthase.
Vance S, Tkachenko O, Thomas B, Bassuni M, Hong H, Nietlispach D, Broadhurst W
Biochem J 473 1097-110 (2016)
PMID: 26920023

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179916 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type I modular polyketide synthase Chain: A
Molecule details ›
Chain: A
Length: 95 amino acids
Theoretical weight: 10.24 KDa
Source organism: Mycobacterium ulcerans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6MZA5 (Residues: 2050-2140; Coverage: 4%)
Gene names: MUP039c, mlsA2
Sequence domains: Phosphopantetheine attachment site
Structure domains: ACP-like

Ligands and Environments

1 bound ligand:
Ligand 66S 1 x 66S
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 80%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

The Uncommon Enzymology of Cis-Acyltransferase Assembly Lines.
Keatinge-Clay AT. (2017)
4 more

1 mention without citation

Sticky swinging arm dynamics: studies of an acyl carrier protein domain from the mycolactone polyketide synthase.
Vance et al. (2016)