› 5hv1

X-ray diffraction
3.1Å resolution

Rifampin phosphotransferase in complex with AMPPNP and rifampin from Listeria monocytogenes

Released:
DOI: 10.2210/pdb5hv1/pdb
PDB 5hv1 coloured by chain and viewed from the front.
PDB 5hv1 coloured by chain and viewed from the side.
PDB 5hv1 coloured by chain and viewed from the top.
Source organism: Listeria monocytogenes
Primary publication:
Structural basis of rifampin inactivation by rifampin phosphotransferase.
Qi X, Lin W, Ma M, Wang C, He Y, He N, Gao J, Zhou H, Xiao Y, Wang Y, Zhang P
Proc. Natl. Acad. Sci. U.S.A. (2016)
PMID: 27001859

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate synthase Chain: A
Molecule details ›
Chain: A
Length: 883 amino acids
Theoretical weight: 98.81 KDa
Source organism: Listeria monocytogenes
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
Ligand ANP 1 x ANP
Ligand RFP 1 x RFP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P6522
Unit cell:
a: 151.09Å b: 151.09Å c: 191.648Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.253 0.25 0.286
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

The Structure of the Antibiotic Deactivating, N-hydroxylating Rifampicin Monooxygenase.
Liu et al. (2016)
7 more

1 mention without citation

Structural basis of rifampin inactivation by rifampin phosphotransferase.
Qi et al. (2016)