Electron Microscopy
6.6Å resolution

Structural basis of backwards motion in kinesin-14: minus-end directed nKn664 in the nucleotide-free state


Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1B chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.11 KDa
Source organism: Bos taurus
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Tubulin beta-2B chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 49.91 KDa
Source organism: Bos taurus
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Kinesin-like protein; Protein claret segregational Chain: K
Molecule details ›
Chain: K
Length: 371 amino acids
Theoretical weight: 41.62 KDa
Source organisms: Expression system: Escherichia coli
  • Canonical: P20480 (Residues: 325-349, 510-511, 513-515, 664-700; Coverage: 10%)
  • Canonical: G3V6L4 (Residues: 10-160, 163-164, 168-320; Coverage: 32%)
Gene names: CA(ND), CG7831, Kif5c, ncd

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.6Å
Relevant EMDB volumes: EMD-8059
Expression system: Escherichia coli