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Crystal structure of bile salt hydrolase from Lactobacillus salivarius.

Xu F, Guo F, Hu XJ, Lin J
Acta Crystallogr F Struct Biol Commun 72 376-81 (2016)
Cited: 16 times
EuropePMC logo PMID: 27139829

Abstract

Bile salt hydrolase (BSH) is a gut-bacterial enzyme that negatively influences host fat digestion and energy harvesting. The BSH enzyme activity functions as a gateway reaction in the small intestine by the deconjugation of glycine-conjugated or taurine-conjugated bile acids. Extensive gut-microbiota studies have suggested that BSH is a key mechanistic microbiome target for the development of novel non-antibiotic food additives to improve animal feed production and for the design of new measures to control obesity in humans. However, research on BSH is still in its infancy, particularly in terms of the structural basis of BSH function, which has hampered the development of BSH-based strategies for improving human and animal health. As an initial step towards the structure-function analysis of BSH, C-terminally His-tagged BSH from Lactobacillus salivarius NRRL B-30514 was crystallized in this study. The 1.90 Å resolution crystal structure of L. salivarius BSH was determined by molecular replacement using the structure of Clostridium perfringens BSH as a starting model. It revealed this BSH to be a member of the N-terminal nucleophile hydrolase superfamily. Crystals of apo BSH belonged to space group P21212, with unit-cell parameters a = 90.79, b = 87.35, c = 86.76 Å (PDB entry 5hke). Two BSH molecules packed perfectly as a dimer in one asymmetric unit. Comparative structural analysis of L. salivarius BSH also identified potential residues that contribute to catalysis and substrate specificity.

Reviews - 5hke mentioned but not cited (3)

  1. Review: microbial transformations of human bile acids. Guzior DV, Quinn RA. Microbiome 9 140 (2021)
  2. Bile salt hydrolases: Structure and function, substrate preference, and inhibitor development. Dong Z, Lee BH. Protein Sci 27 1742-1754 (2018)
  3. A structural metagenomics pipeline for examining the gut microbiome. Walker ME, Simpson JB, Redinbo MR. Curr Opin Struct Biol 75 102416 (2022)

Articles - 5hke mentioned but not cited (5)

  1. Bile salt hydrolases: Gatekeepers of bile acid metabolism and host-microbiome crosstalk in the gastrointestinal tract. Foley MH, O'Flaherty S, Barrangou R, Theriot CM. PLoS Pathog 15 e1007581 (2019)
  2. Characterization of Bile Salt Hydrolase from Lactobacillus gasseri FR4 and Demonstration of Its Substrate Specificity and Inhibitory Mechanism Using Molecular Docking Analysis. Rani RP, Anandharaj M, Ravindran AD. Front Microbiol 8 1004 (2017)
  3. Crystal structure of bile salt hydrolase from Lactobacillus salivarius. Xu F, Guo F, Hu XJ, Lin J. Acta Crystallogr F Struct Biol Commun 72 376-381 (2016)
  4. Identifying a Novel Bile Salt Hydrolase from the Keystone Gut Bacterium Christensenella minuta. Déjean G, Tudela H, Bruno L, Kissi D, Rawadi G, Claus SP. Microorganisms 9 1252 (2021)
  5. The complex structure of bile salt hydrolase from Lactobacillus salivarius reveals the structural basis of substrate specificity. Xu F, Hu XJ, Singh W, Geng W, Tikhonova IG, Lin J. Sci Rep 9 12438 (2019)


Reviews citing this publication (4)

  1. Interactions between gut bacteria and bile in health and disease. Long SL, Gahan CGM, Joyce SA. Mol Aspects Med 56 54-65 (2017)
  2. Bile Salt Hydrolases: At the Crossroads of Microbiota and Human Health. Bourgin M, Kriaa A, Mkaouar H, Mariaule V, Jablaoui A, Maguin E, Rhimi M. Microorganisms 9 1122 (2021)
  3. Functional and Phylogenetic Diversity of BSH and PVA Enzymes. Daly JW, Keely SJ, Gahan CGM. Microorganisms 9 732 (2021)
  4. Regulation of gut microbiota-bile acids axis by probiotics in inflammatory bowel disease. Li L, Liu T, Gu Y, Wang X, Xie R, Sun Y, Wang B, Cao H. Front Immunol 13 974305 (2022)

Articles citing this publication (4)

  1. A sulfated polysaccharide from Gracilaria Lemaneiformis regulates cholesterol and bile acid metabolism in high-fat diet mice. Huang S, Pang D, Li X, You L, Zhao Z, Cheung PC, Zhang M, Liu D. Food Funct 10 3224-3236 (2019)
  2. Bile salt hydrolases shape the bile acid landscape and restrict Clostridioides difficile growth in the murine gut. Foley MH, Walker ME, Stewart AK, O'Flaherty S, Gentry EC, Patel S, Beaty VV, Allen G, Pan M, Simpson JB, Perkins C, Vanhoy ME, Dougherty MK, McGill SK, Gulati AS, Dorrestein PC, Baker ES, Redinbo MR, Barrangou R, Theriot CM. Nat Microbiol 8 611-628 (2023)
  3. Asparagine 79 is an important amino acid for catalytic activity and substrate specificity of bile salt hydrolase (BSH). Öztürk M, Önal C. Mol Biol Rep 46 4361-4368 (2019)
  4. Isolation of a Highly Thermostable Bile Salt Hydrolase With Broad Substrate Specificity From Lactobacillus paragasseri. Kusada H, Arita M, Tohno M, Tamaki H. Front Microbiol 13 810872 (2022)


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