› 5hke

X-ray diffraction
1.9Å resolution

bile salt hydrolase from Lactobacillus salivarius

Released:
DOI: 10.2210/pdb5hke/pdb
PDB entry 5hke coloured by chain, front view.
PDB entry 5hke coloured by chain, side view.
PDB entry 5hke coloured by chain, top view.
Source organism: Ligilactobacillus salivarius
Primary publication:
Crystal structure of bile salt hydrolase from Lactobacillus salivarius.
Xu F, Guo F, Hu XJ, Lin J
Acta Crystallogr F Struct Biol Commun 72 376-81 (2016)
PMID: 27139829

Function and Biology Details

Reaction catalysed: 
3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholan-24-oylglycine + H(2)O = 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + glycine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-125335 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Choloylglycine hydrolase/NAAA C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 333 amino acids
Theoretical weight: 37.93 KDa
Source organism: Ligilactobacillus salivarius
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: J7H3P9 (Residues: 1-324; Coverage: 100%)
Sequence domains: Linear amide C-N hydrolases, choloylglycine hydrolase family
Structure domains: Penicillin V Acylase; Chain A

Ligands and Environments

1 bound ligand:
Ligand PO4 4 x PO4
1 modified residue:
Ligand OCS 2 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21212
Unit cell:
a: 90.79Å b: 87.356Å c: 86.766Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.152 0.185
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Interactions between gut bacteria and bile in health and disease.
Long et al. (2017)
3 more

8 mentions without citation

Review: microbial transformations of human bile acids.
Guzior et al. (2021)
7 more