5hk3

X-ray diffraction
1.56Å resolution

Crystal structure of M. tuberculosis MazF-mt3 T52D-F62D mutant in complex with DNA

Released:
Entry authors: Yen TJ, Brennan RG

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Endoribonuclease MazF6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 114 amino acids
Theoretical weight: 12.26 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WII3 (Residues: 1-114; Coverage: 100%)
Gene names: MTCY39.28, Rv1991c, mazF-mt3, mazF6
Sequence domains: PemK-like, MazF-like toxin of type II toxin-antitoxin system
Structure domains: SH3 type barrels.
DNA (5'-D(*AP*GP*UP*CP*UP*CP*CP*UP*UP*UP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.21 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 47.311Å b: 64.244Å c: 73.915Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.192 0.207
Expression system: Escherichia coli