X-ray diffraction
2.34Å resolution

Crystal structure of glycine sarcosine N-methyltransferase from Methanohalophilus portucalensis in complex with S-adenosylhomocysteine and dimethylglycine


Function and Biology Details

Reaction catalysed:
(1a) S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Methyltransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 284 amino acids
Theoretical weight: 32.8 KDa
Source organism: Methanohalophilus portucalensis FDF-1
Expression system: Escherichia coli BL21(DE3)
  • Canonical: F6KV61 (Residues: 1-263; Coverage: 100%)
Gene names: EFE41_00900, MPF_0823, SAMN06264941_0527, gsmt
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

Cofactor: Ligand SAH 1 x SAH
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: I222
Unit cell:
a: 51.975Å b: 121.219Å c: 131.686Å
α: 90° β: 90° γ: 90°
R R work R free
0.159 0.156 0.207
Expression system: Escherichia coli BL21(DE3)