5h9u

X-ray diffraction
2.67Å resolution

Crystal structure of a thermostable methionine adenosyltransferase

Released:
Source organism: Thermus thermophilus HB27
Entry authors: Feng Y, Wang W

Function and Biology Details

Reaction catalysed:
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 413 amino acids
Theoretical weight: 45.2 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72I53 (Residues: 1-395; Coverage: 100%)
Gene names: TT_C1279, metK
Sequence domains:
Structure domains: GMP Synthetase; Chain A, domain 3

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 230.024Å b: 77.053Å c: 96.792Å
α: 90° β: 112.04° γ: 90°
R-values:
R R work R free
0.176 0.173 0.233
Expression system: Escherichia coli