Assemblies
Assembly Name:
Cascade complex and RNA and DNA
Multimeric state:
hetero tetradecamer
Accessible surface area:
129260.67 Å2
Buried surface area:
64520.38 Å2
Dissociation area:
1,441.45
Å2
Dissociation energy (ΔGdiss):
0.01
kcal/mol
Dissociation entropy (TΔSdiss):
13.34
kcal/mol
Symmetry number:
1
PDBe Complex ID:
PDB-CPX-118730
Macromolecules
Chain: A
Length: 502 amino acids
Theoretical weight: 55.96 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
Pfam: CRISPR-associated protein Cse1 (CRISPR_cse1)
InterPro: CRISPR-associated protein Cse1
Length: 502 amino acids
Theoretical weight: 55.96 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
- Canonical: Q46901 (Residues: 1-502; Coverage: 100%)
Pfam: CRISPR-associated protein Cse1 (CRISPR_cse1)
InterPro: CRISPR-associated protein Cse1
Chains: B, C
Length: 165 amino acids
Theoretical weight: 19.23 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
Pfam: CRISPR-associated protein Cse2 (CRISPR_cse2)
InterPro:
CATH: CRISPR-associated protein Cse2
Length: 165 amino acids
Theoretical weight: 19.23 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
- Canonical: P76632 (Residues: 1-160; Coverage: 100%)
Pfam: CRISPR-associated protein Cse2 (CRISPR_cse2)
InterPro:
CATH: CRISPR-associated protein Cse2
Chains: D, E, F, G, H, I
Length: 363 amino acids
Theoretical weight: 40.07 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
Pfam: CT1975-like protein
InterPro: CRISPR-associated protein, CT1975
Length: 363 amino acids
Theoretical weight: 40.07 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
- Canonical: Q46899 (Residues: 1-363; Coverage: 100%)
Pfam: CT1975-like protein
InterPro: CRISPR-associated protein, CT1975
Chain: J
Length: 224 amino acids
Theoretical weight: 25.24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
Pfam: CRISPR-associated protein (Cas_Cas5)
InterPro:
Length: 224 amino acids
Theoretical weight: 25.24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
- Canonical: Q46898 (Residues: 1-224; Coverage: 100%)
Pfam: CRISPR-associated protein (Cas_Cas5)
InterPro:
- CRISPR-associated protein, CasD
- CRISPR-associated protein Cas5, N-terminal
- CRISPR-associated protein, Cas5
Chain: K
Length: 199 amino acids
Theoretical weight: 22.32 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
Pfam: CRISPR associated protein
InterPro: CRISPR-associated protein Cse3
CATH: Crispr-associated protein; domain 2
Length: 199 amino acids
Theoretical weight: 22.32 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
- Canonical: Q46897 (Residues: 1-199; Coverage: 100%)
Pfam: CRISPR associated protein
InterPro: CRISPR-associated protein Cse3
CATH: Crispr-associated protein; domain 2
Name:
DNA (28-MER) Non-target
Representative chains: M
Length: 28 nucleotides
Theoretical weight: 8.6 KDa
Representative chains: M
Length: 28 nucleotides
Theoretical weight: 8.6 KDa
Name:
DNA (50-MER) Target
Representative chains: N
Length: 50 nucleotides
Theoretical weight: 15.39 KDa
Representative chains: N
Length: 50 nucleotides
Theoretical weight: 15.39 KDa