5h9f

X-ray diffraction
2.45Å resolution

Crystal structure of E. coli Cascade bound to a PAM-containing dsDNA target at 2.45 angstrom resolution.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
CRISPR system Cascade subunit CasA Chain: A
Molecule details ›
Chain: A
Length: 502 amino acids
Theoretical weight: 55.96 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46901 (Residues: 1-502; Coverage: 100%)
Gene names: JW2730, b2760, casA, cse1, ygcL
Sequence domains: CRISPR-associated protein Cse1 (CRISPR_cse1)
CRISPR system Cascade subunit CasB Chains: B, C
Molecule details ›
Chains: B, C
Length: 165 amino acids
Theoretical weight: 19.23 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P76632 (Residues: 1-160; Coverage: 100%)
Gene names: JW2729, b2759, casB, cse2, ygcK
Sequence domains: CRISPR-associated protein Cse2 (CRISPR_cse2)
Structure domains: CRISPR-associated protein Cse2
CRISPR system Cascade subunit CasC Chains: D, E, F, G, H, I
Molecule details ›
Chains: D, E, F, G, H, I
Length: 363 amino acids
Theoretical weight: 40.07 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46899 (Residues: 1-363; Coverage: 100%)
Gene names: JW2728, b2758, cas4, casC, cse4, ygcJ
Sequence domains: CT1975-like protein
CRISPR system Cascade subunit CasD Chain: J
Molecule details ›
Chain: J
Length: 224 amino acids
Theoretical weight: 25.24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46898 (Residues: 1-224; Coverage: 100%)
Gene names: JW5844, b2757, cas5, casD, ygcI
Sequence domains: CRISPR-associated protein (Cas_Cas5)
Structure domains: Alpha-Beta Plaits
CRISPR system Cascade subunit CasE Chain: K
Molecule details ›
Chain: K
Length: 199 amino acids
Theoretical weight: 22.32 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46897 (Residues: 1-199; Coverage: 100%)
Gene names: JW2726, b2756, cas6e, casE, ygcH
Sequence domains: CRISPR associated protein
Structure domains: Crispr-associated protein; domain 2
DNA (28-MER) Non-target Chain: M
Molecule details ›
Chain: M
Length: 28 nucleotides
Theoretical weight: 8.6 KDa
DNA (50-MER) Target Chain: N
Molecule details ›
Chain: N
Length: 50 nucleotides
Theoretical weight: 15.39 KDa
crRNA (61-MER) Chain: L
Molecule details ›
Chain: L
Length: 61 nucleotides
Theoretical weight: 19.68 KDa

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Unit cell:
a: 92.979Å b: 150.059Å c: 400.546Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.233
Expression system: Escherichia coli