› 5h8l

X-ray diffraction
2.29Å resolution

Crystal structure of Medicago truncatula N-carbamoylputrescine amidohydrolase (MtCPA) C158S mutant in complex with putrescine

Released:
DOI: 10.2210/pdb5h8l/pdb
PDB entry 5h8l coloured by chain, front view.
PDB entry 5h8l coloured by chain, side view.
PDB entry 5h8l coloured by chain, top view.
Source organism: Medicago truncatula
Primary publication:
Structural Investigations of N-carbamoylputrescine Amidohydrolase from Medicago truncatula: Insights into the Ultimate Step of Putrescine Biosynthesis in Plants.
Sekula B, Ruszkowski M, Malinska M, Dauter Z
Front Plant Sci 7 350 (2016)
PMID: 27066023

Function and Biology Details

Reaction catalysed: 
N-carbamoylputrescine + H(2)O = putrescine + CO(2) + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124569 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CN hydrolase domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 304 amino acids
Theoretical weight: 34.11 KDa
Source organism: Medicago truncatula
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G7ITU5 (Residues: 1-301; Coverage: 100%)
Gene names: 11406591, MTR_2g086600, MtrunA17_Chr2g0321561
Sequence domains: Carbon-nitrogen hydrolase
Structure domains: Carbon-nitrogen hydrolase

Ligands and Environments

4 bound ligands:
Ligand GOL 30 x GOL
Ligand EDO 17 x EDO
Ligand PUT 12 x PUT
Ligand PEG 3 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21212
Unit cell:
a: 152.21Å b: 211.082Å c: 208.613Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.154 0.206
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Putrescine: A Key Metabolite Involved in Plant Development, Tolerance and Resistance Responses to Stress.
González-Hernández et al. (2022)
12 other articles

1 mention without citation

Structural Investigations of N-carbamoylputrescine Amidohydrolase from Medicago truncatula: Insights into the Ultimate Step of Putrescine Biosynthesis in Plants.
Sekula et al. (2016)