5h3u

X-ray diffraction
2.5Å resolution

Sm RNA bound to GEMIN5-WD

Released:
Source organism: Homo sapiens
Primary publication:
Structural basis for specific recognition of pre-snRNA by Gemin5.
Cell Res 26 1353-1356 (2016)
PMID: 27834343

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
PDBe Complex ID:
PDB-CPX-186252 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Gem-associated protein 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 740 amino acids
Theoretical weight: 82.3 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8TEQ6 (Residues: 1-740; Coverage: 49%)
Gene name: GEMIN5
Sequence domains:
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
RNA (5'-R(*AP*AP*UP*UP*UP*UP*UP*GP*AP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 10 nucleotides
Theoretical weight: 3.12 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 76.836Å b: 92.083Å c: 110.801Å
α: 90° β: 99.99° γ: 90°
R-values:
R R work R free
0.204 0.202 0.256
Expression systems:
  • Spodoptera frugiperda
  • Not provided