PDBe 5h2x

X-ray diffraction
2.2Å resolution

Crystal structure of the karyopherin Kap60p bound to the SUMO protease Ulp1p (150-172)

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of the alpha-linked peptide bond in the sequence Gly-Gly-|-Ala-Thr-Tyr at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptide, Smt3, leading to the mature form of the protein. A second reaction involves the cleavage of an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 423 amino acids
Theoretical weight: 46.85 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02821 (Residues: 88-510; Coverage: 78%)
Gene names: KAP60, N1606, SRP1, YNL189W
Sequence domains: Armadillo/beta-catenin-like repeat
Structure domains: Leucine-rich Repeat Variant
Ubiquitin-like-specific protease 1 Chain: B
Molecule details ›
Chain: B
Length: 23 amino acids
Theoretical weight: 2.81 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02724 (Residues: 150-172; Coverage: 4%)
Gene names: LPB11C, ULP1, YPL020C

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 54.57Å b: 77.48Å c: 114.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.202 0.228
Expression system: Escherichia coli