› 5h1k

X-ray diffraction
1.9Å resolution

Crystal structure of WD40 repeat domains of Gemin5 in complex with 13-nt U4 snRNA fragment

Released:
DOI: 10.2210/pdb5h1k/pdb
PDB entry 5h1k coloured by chain, front view.
PDB entry 5h1k coloured by chain, side view.
PDB entry 5h1k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for snRNA recognition by the double-WD40 repeat domain of Gemin5.
Jin W, Wang Y, Liu CP, Yang N, Jin M, Cong Y, Wang M, Xu RM
Genes Dev 30 2391-2403 (2016)
PMID: 27881601

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Gem-associated protein 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 734 amino acids
Theoretical weight: 81.43 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8TEQ6 (Residues: 1-726; Coverage: 48%)
Gene name: GEMIN5
Sequence domains:
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
U4 snRNA (5'-R(*GP*CP*AP*AP*UP*UP*UP*UP*UP*GP*AP*CP*A)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 13 nucleotides
Theoretical weight: 4.1 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 98.654Å b: 107.191Å c: 151.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.206
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

6 review citations

Long Noncoding RNA in Cancer: Wiring Signaling Circuitry.
Lin et al. (2018)
5 more

3 mentions without citation

Control of the eIF4E activity: structural insights and pharmacological implications.
Romagnoli et al. (2021)
2 more