› 5gzz

X-ray diffraction
2.39Å resolution

Crystal Structure of FIN219-SjGST complex with JA

Released:
DOI: 10.2210/pdb5gzz/pdb
PDB entry 5gzz coloured by chain, front view.
PDB entry 5gzz coloured by chain, side view.
PDB entry 5gzz coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of jasmonate-amido synthetase FIN219 in complex with glutathione S-transferase FIP1 during the JA signal regulation.
Chen CY, Ho SS, Kuo TY, Hsieh HL, Cheng YS
Proc Natl Acad Sci U S A 114 E1815-E1824 (2017)
PMID: 28223489

Function and Biology Details

Reactions catalysed: 
ATP + jasmonate + an L-amino acid = AMP + diphosphate + a jasmonoyl-L-amino acid
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-140161 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Jasmonoyl--L-amino acid synthetase JAR1 Chain: A
Molecule details ›
Chain: A
Length: 575 amino acids
Theoretical weight: 64.42 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9SKE2 (Residues: 1-575; Coverage: 100%)
Gene names: At2g46370, F11C10.6, FIN219, JAR1
Sequence domains: GH3 auxin-responsive promoter
Glutathione S-transferase class-mu 26 kDa isozyme Chains: B, C, D, E, F, G
Molecule details ›
Chains: B, C, D, E, F, G
Length: 218 amino acids
Theoretical weight: 25.53 KDa
Source organism: Schistosoma japonicum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08515 (Residues: 1-218; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GSH 6 x GSH
1 bound ligand:
Ligand JAA 1 x JAA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P1
Unit cell:
a: 57.274Å b: 91.802Å c: 91.728Å
α: 89.99° β: 89.99° γ: 89.93°
R-values:
R R work R free
0.207 0.204 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Jasmonates: News on Occurrence, Biosynthesis, Metabolism and Action of an Ancient Group of Signaling Compounds.
Wasternack et al. (2018)
3 more

1 mention without citation

Structural basis of jasmonate-amido synthetase FIN219 in complex with glutathione S-transferase FIP1 during the JA signal regulation.
Chen et al. (2017)