› 5gy3

X-ray diffraction
1.77Å resolution

The crystal structure of endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae

Released:
DOI: 10.2210/pdb5gy3/pdb
PDB entry 5gy3 coloured by chain, front view.
PDB entry 5gy3 coloured by chain, side view.
PDB entry 5gy3 coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
The crystal structure of the endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae.
Attigani A, Sun L, Wang Q, Liu Y, Bai D, Li S, Huang X
Acta Crystallogr F Struct Biol Commun 72 870-876 (2016)
PMID: 27917834

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102209 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucanase Chain: A
Molecule details ›
Chain: A
Length: 310 amino acids
Theoretical weight: 35.3 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0J4VP90 (Residues: 24-333; Coverage: 93%)
Gene names: AN2335V1_3670, IAP99_01170, NCTC9177_00719, SAMEA3729809_04098
Sequence domains: Glycosyl hydrolases family 8
Structure domains: Glycosyltransferase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 53.57Å b: 73.256Å c: 79.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.163 0.195
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

Enterococcus faecalis α1-2-mannosidase (EfMan-I): an efficient catalyst for glycoprotein N-glycan modification.
Li et al. (2020)
4 more

2 mentions without citation

The crystal structure of the endoglucanase Cel10, a family 8 glycosyl hydrolase from Klebsiella pneumoniae.
Attigani et al. (2016)
1 more