› 5gxz

X-ray diffraction
2.05Å resolution

Crystal structure of endoglucanase CelQ from Clostridium thermocellum complexed with cellobiose and cellotriose

Released:
DOI: 10.2210/pdb5gxz/pdb
PDB entry 5gxz coloured by chain, front view.
PDB entry 5gxz coloured by chain, side view.
PDB entry 5gxz coloured by chain, top view.
Source organism: Acetivibrio thermocellus
Primary publication:
Crystal Structures of the C-Terminally Truncated Endoglucanase Cel9Q from Clostridium thermocellum Complexed with Cellodextrins and Tris.
Jeng WY, Liu CI, Lu TJ, Lin HJ, Wang NC, Wang AH
Chembiochem 20 295-307 (2019)
PMID: 30609216

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189567 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Glucanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 610 amino acids
Theoretical weight: 69.21 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AJF8 (Residues: 28-628; Coverage: 88%)
Gene name: celQ
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
4 bound ligands:
Ligand CA 4 x CA
Ligand CL 6 x CL
Ligand BR 6 x BR
Ligand 7PE 1 x 7PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P212121
Unit cell:
a: 108.045Å b: 108.668Å c: 139.625Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Contribution of calcium ligands in substrate binding and product release in the Acetovibrio thermocellus glycoside hydrolase family 9 cellulase CelR.
Kuch et al. (2023)