X-ray diffraction
1.7Å resolution

Crystal structure of Acryloyl-CoA reductase AcuI in complex with NADPH

Source organism: Ruegeria pomeroyi DSS-3
Entry authors: Zhang YZ, Wang P, Cao HY

Function and Biology Details

Reaction catalysed:
Propanoyl-CoA + NADP(+) = acrylyl-CoA + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Acrylyl-CoA reductase AcuI Chains: A, B
Molecule details ›
Chains: A, B
Length: 331 amino acids
Theoretical weight: 34.62 KDa
Source organism: Ruegeria pomeroyi DSS-3
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q5LS56 (Residues: 1-330; Coverage: 100%)
Gene names: SPO1914, acuI
Sequence domains: Zinc-binding dehydrogenase
Structure domains:

Ligands and Environments

Cofactor: Ligand NAP 2 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P21212
Unit cell:
a: 79.903Å b: 111.277Å c: 74.526Å
α: 90° β: 90° γ: 90°
R R work R free
0.169 0.168 0.198
Expression system: Escherichia coli BL21(DE3)