› 5gxe

X-ray diffraction
1.7Å resolution

Crystal structure of Acryloyl-CoA reductase AcuI in complex with NADPH

Released:
DOI: 10.2210/pdb5gxe/pdb
PDB 5gxe coloured by chain and viewed from the front.
PDB 5gxe coloured by chain and viewed from the side.
PDB 5gxe coloured by chain and viewed from the top.
Source organism: Ruegeria pomeroyi DSS-3
Entry authors: Zhang YZ, Wang P, Cao HY

Function and Biology Details

Reaction catalysed: 
Propanoyl-CoA + NADP(+) = acrylyl-CoA + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acrylyl-CoA reductase AcuI Chains: A, B
Molecule details ›
Chains: A, B
Length: 331 amino acids
Theoretical weight: 34.62 KDa
Source organism: Ruegeria pomeroyi DSS-3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5LS56 (Residues: 1-330; Coverage: 100%)
Gene names: SPO1914, acuI
Sequence domains: Zinc-binding dehydrogenase
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P21212
Unit cell:
a: 79.903Å b: 111.277Å c: 74.526Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.168 0.198
Expression system: Escherichia coli BL21(DE3)

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