› 5gvw

X-ray diffraction
2.4Å resolution

Crystal structure of the apo-form glycosyltransferase GlyE in Streptococcus pneumoniae TIGR4

Released:
DOI: 10.2210/pdb5gvw/pdb
PDB entry 5gvw coloured by chain, front view.
PDB entry 5gvw coloured by chain, side view.
PDB entry 5gvw coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Primary publication:
Defining the enzymatic pathway for polymorphic O-glycosylation of the pneumococcal serine-rich repeat protein PsrP.
Jiang YL, Jin H, Yang HB, Zhao RL, Wang S, Chen Y, Zhou CZ
J Biol Chem 292 6213-6224 (2017)
PMID: 28246170

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165697 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl transferase family 8 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 406 amino acids
Theoretical weight: 47.46 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 89.119Å b: 84.211Å c: 130.113Å
α: 90° β: 89.97° γ: 90°
R-values:
R R work R free
0.201 0.198 0.255
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Serine-rich repeat proteins from gut microbes.
Latousakis et al. (2020)
3 more

1 mention without citation

Defining the enzymatic pathway for polymorphic O-glycosylation of the pneumococcal serine-rich repeat protein PsrP.
Jiang et al. (2017)