› 5gte

X-ray diffraction
2Å resolution

Crystal structure of onion lachrymatory factor synthase (LFS), solute-free form

Released:
DOI: 10.2210/pdb5gte/pdb
PDB entry 5gte coloured by chain, front view.
PDB entry 5gte coloured by chain, side view.
PDB entry 5gte coloured by chain, top view.
Source organism: Allium cepa
Primary publication:
Dissecting the Stereocontrolled Conversion of Short-Lived Sulfenic Acid by Lachrymatory Factor Synthase
Arakawa T, Sato Y, Yamada M, Takabe J, Moriwaki Y, Masamura N, Kato M, Aoyagi M, Kamoi T, Terada T, Shimizu K, Tsuge N, Imai S, Fushinobu S
ACS Catalysis 10 9-19 (2020)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157773 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lachrymatory-factor synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 175 amino acids
Theoretical weight: 19.91 KDa
Source organism: Allium cepa
Expression system: Escherichia coli
UniProt:
  • Canonical: P59082 (Residues: 1-169; Coverage: 100%)
Gene name: LFS
Sequence domains: Polyketide cyclase / dehydrase and lipid transport

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P212121
Unit cell:
a: 65.123Å b: 118.656Å c: 124.504Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.233
Expression system: Escherichia coli

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