PDBe 5gtd

X-ray diffraction
2.69Å resolution

o-Succinylbenzoate CoA Synthetase (MenE) from Bacillus Subtilis in Complex with the Acyl-adenylate Intermediate OSB-AMP

Released:

Function and Biology Details

Reaction catalysed:
ATP + 2-succinylbenzoate + CoA = AMP + diphosphate + 2-succinylbenzoyl-CoA
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-succinylbenzoate--CoA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 494 amino acids
Theoretical weight: 55.27 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P23971 (Residues: 1-486; Coverage: 100%)
Gene names: BSU30790, menE
Sequence domains:
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43
Unit cell:
a: 121.814Å b: 121.814Å c: 98.563Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.2 0.237
Expression system: Escherichia coli