X-ray diffraction
1.7Å resolution

The X-ray structure of 7beta-hydroxysteroid dehydrogenase

Primary publication:
Structure of NADP(+)-bound 7β-hydroxysteroid dehydrogenase reveals two cofactor-binding modes.
Acta Crystallogr F Struct Biol Commun 73 246-252 (2017)
PMID: 28471355

Function and Biology Details

Reaction catalysed:
A 7-beta-hydroxysteroid + NADP(+) = a 7-oxosteroid + NADPH
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
7beta-hydroxysteroid dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 263 amino acids
Theoretical weight: 28.78 KDa
Source organism: Collinsella aerofaciens ATCC 25986
Expression system: Escherichia coli BL21(DE3)
  • Canonical: A4ECA9 (Residues: 1-263; Coverage: 100%)
Gene name: COLAER_02088
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

Cofactor: Ligand NAP 2 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41212
Unit cell:
a: 72.73Å b: 72.73Å c: 171.31Å
α: 90° β: 90° γ: 90°
R R work R free
0.167 0.165 0.201
Expression system: Escherichia coli BL21(DE3)