5grl

X-ray diffraction
2.79Å resolution

Crystal structure of the alpha gamma heterodimer of human IDH3 in complex with Mg(2+), isocitrate and ADP

Released:

Function and Biology Details

Reaction catalysed:
Isocitrate + NAD(+) = 2-oxoglutarate + CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 339 amino acids
Theoretical weight: 36.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P50213 (Residues: 28-366; Coverage: 93%)
Gene name: IDH3A
Sequence domains: Isocitrate/isopropylmalate dehydrogenase
Structure domains: Isopropylmalate Dehydrogenase
Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial Chain: B
Molecule details ›
Chain: B
Length: 354 amino acids
Theoretical weight: 38.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P51553 (Residues: 40-393; Coverage: 90%)
Gene name: IDH3G
Sequence domains: Isocitrate/isopropylmalate dehydrogenase
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P3121
Unit cell:
a: 111.238Å b: 111.238Å c: 145.478Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.226 0.274
Expression system: Escherichia coli BL21(DE3)