5gr6

X-ray diffraction
2Å resolution

Crystal structure of branching enzyme Y500A/D501A double mutant from Cyanothece sp. ATCC 51142

Released:
Source organism: Cyanothece sp. ATCC 51142
Entry authors: Suzuki R, Suzuki E

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1,4-alpha-glucan branching enzyme GlgB Chain: A
Molecule details ›
Chain: A
Length: 793 amino acids
Theoretical weight: 92.55 KDa
Source organism: Cyanothece sp. ATCC 51142
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1WPM8 (Residues: 1-773; Coverage: 100%)
Gene names: cce_2248, glgB, glgB1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P41212
Unit cell:
a: 133.596Å b: 133.596Å c: 184.227Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.194
Expression system: Escherichia coli BL21(DE3)