5gpn

Electron Microscopy
5.4Å resolution

Architecture of mammalian respirasome

Released:
Source organisms:
Primary publication:
The architecture of the mammalian respirasome.
Nature 537 639-43 (2016)
PMID: 27654917
Related structures: EMD-9534

Function and Biology Details

Reactions catalysed:
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
NADH + ubiquinone + 5 H(+)(Side 1) = NAD(+) + ubiquinol + 4 H(+)(Side 2)
4 ferrocytochrome c + O(2) + 8 H(+)(Side 1) = 4 ferricytochrome c + 4 H(2)O(Side 2)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 84-mer (preferred)
PDBe Complex ID:
PDB-CPX-236796 (preferred)
Entry contents:
63 distinct polypeptide molecules
Macromolecules (63 distinct):
Cytochrome b-c1 complex subunit 1, mitochondrial Chains: A, M
Molecule details ›
Chains: A, M
Length: 446 amino acids
Theoretical weight: 49.27 KDa
Source organism: Bos taurus
Cytochrome b-c1 complex subunit 2, mitochondrial Chains: B, N
Molecule details ›
Chains: B, N
Length: 439 amino acids
Theoretical weight: 46.58 KDa
Source organism: Bos taurus
Cytochrome b Chains: C, O
Molecule details ›
Chains: C, O
Length: 379 amino acids
Theoretical weight: 42.62 KDa
Source organism: Bos taurus
Cytochrome c1, heme protein, mitochondrial Chains: D, P
Molecule details ›
Chains: D, P
Length: 241 amino acids
Theoretical weight: 27.32 KDa
Source organism: Bos taurus
Cytochrome b-c1 complex subunit Rieske, mitochondrial Chains: E, Q
Molecule details ›
Chains: E, Q
Length: 274 amino acids
Theoretical weight: 29.57 KDa
Source organism: Bos taurus
Cytochrome b-c1 complex subunit 7 Chains: F, R
Molecule details ›
Chains: F, R
Length: 110 amino acids
Theoretical weight: 13.37 KDa
Source organism: Bos taurus
Cytochrome b-c1 complex subunit 8 Chains: G, S
Molecule details ›
Chains: G, S
Length: 81 amino acids
Theoretical weight: 9.61 KDa
Source organism: Bos taurus
Cytochrome b-c1 complex subunit 6, mitochondrial Chains: H, T
Molecule details ›
Chains: H, T
Length: 78 amino acids
Theoretical weight: 9.19 KDa
Source organism: Bos taurus
Cytochrome b-c1 complex subunit 9 Chains: I, U
Molecule details ›
Chains: I, U
Length: 78 amino acids
Theoretical weight: 7.96 KDa
Source organism: Bos taurus
Cytochrome b-c1 complex subunit 9 Chains: J, L
Molecule details ›
Chains: J, L
Length: 62 amino acids
Theoretical weight: 7.21 KDa
Source organism: Bos taurus
Cytochrome b-c1 complex subunit 10 Chains: K, V
Molecule details ›
Chains: K, V
Length: 56 amino acids
Theoretical weight: 6.53 KDa
Source organism: Bos taurus
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 Chain: W
Molecule details ›
Chain: W
Length: 264 amino acids
Theoretical weight: 30.24 KDa
Source organism: Sus scrofa
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial Chain: Y
Molecule details ›
Chain: Y
Length: 727 amino acids
Theoretical weight: 79.63 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial Chain: Z
Molecule details ›
Chain: Z
Length: 463 amino acids
Theoretical weight: 52.55 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial Chain: a
Molecule details ›
Chain: a
Length: 216 amino acids
Theoretical weight: 23.78 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8 Chain: b
Molecule details ›
Chain: b
Length: 212 amino acids
Theoretical weight: 23.89 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial Chain: c
Molecule details ›
Chain: c
Length: 464 amino acids
Theoretical weight: 50.64 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial Chain: d
Molecule details ›
Chain: d
Length: 249 amino acids
Theoretical weight: 27.44 KDa
Source organism: Sus scrofa
NADH-ubiquinone oxidoreductase chain 1 Chain: e
Molecule details ›
Chain: e
Length: 318 amino acids
Theoretical weight: 35.67 KDa
Source organism: Sus scrofa
NADH-ubiquinone oxidoreductase chain 2 Chain: f
Molecule details ›
Chain: f
Length: 347 amino acids
Theoretical weight: 39.08 KDa
Source organism: Sus scrofa
NADH-ubiquinone oxidoreductase chain 3 Chain: g
Molecule details ›
Chain: g
Length: 115 amino acids
Theoretical weight: 13 KDa
Source organism: Sus scrofa
NADH-ubiquinone oxidoreductase chain 4 Chain: h
Molecule details ›
Chain: h
Length: 459 amino acids
Theoretical weight: 51.86 KDa
Source organism: Sus scrofa
NADH-ubiquinone oxidoreductase chain 4L Chain: i
Molecule details ›
Chain: i
Length: 98 amino acids
Theoretical weight: 10.83 KDa
Source organism: Sus scrofa
NADH-ubiquinone oxidoreductase chain 5 Chain: j
Molecule details ›
Chain: j
Length: 606 amino acids
Theoretical weight: 68.7 KDa
Source organism: Sus scrofa
NADH-ubiquinone oxidoreductase chain 6 Chains: k, l
Molecule details ›
Chains: k, l
Length: 175 amino acids
Theoretical weight: 19.02 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 Chain: m
Molecule details ›
Chain: m
Length: 84 amino acids
Theoretical weight: 9.23 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 Chain: n
Molecule details ›
Chain: n
Length: 99 amino acids
Theoretical weight: 11.1 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 Chain: o
Molecule details ›
Chain: o
Length: 106 amino acids
Theoretical weight: 12.51 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial Chain: p
Molecule details ›
Chain: p
Length: 377 amino acids
Theoretical weight: 42.61 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial Chains: q, v
Molecule details ›
Chains: q, v
Length: 357 amino acids
Theoretical weight: 40.48 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 Chain: r
Molecule details ›
Chain: r
Length: 144 amino acids
Theoretical weight: 16.91 KDa
Source organism: Sus scrofa
Acyl carrier protein Chains: Aa, t
Molecule details ›
Chains: Aa, t
Length: 156 amino acids
Theoretical weight: 17.33 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial Chains: Ai, u
Molecule details ›
Chains: Ai, u
Length: 189 amino acids
Theoretical weight: 21.59 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial Chain: w
Molecule details ›
Chain: w
Length: 175 amino acids
Theoretical weight: 19.72 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial Chain: x
Molecule details ›
Chain: x
Length: 123 amino acids
Theoretical weight: 13.35 KDa
Source organism: Sus scrofa
Cytochrome c oxidase subunit 3 Chain: 0
Molecule details ›
Chain: 0
Length: 261 amino acids
Theoretical weight: 29.94 KDa
Source organism: Bos taurus
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial Chain: 1
Molecule details ›
Chain: 1
Length: 147 amino acids
Theoretical weight: 17.18 KDa
Source organism: Bos taurus
Cytochrome c oxidase subunit 5A, mitochondrial Chain: 2
Molecule details ›
Chain: 2
Length: 109 amino acids
Theoretical weight: 12.45 KDa
Source organism: Bos taurus
Cytochrome c oxidase subunit 5B, mitochondrial Chain: 3
Molecule details ›
Chain: 3
Length: 98 amino acids
Theoretical weight: 10.68 KDa
Source organism: Bos taurus
Cytochrome c oxidase subunit 6A2, mitochondrial Chain: 4
Molecule details ›
Chain: 4
Length: 84 amino acids
Theoretical weight: 9.45 KDa
Source organism: Bos taurus
Cytochrome c oxidase subunit 6B1 Chain: 5
Molecule details ›
Chain: 5
Length: 85 amino acids
Theoretical weight: 10.04 KDa
Source organism: Bos taurus
Cytochrome c oxidase subunit 6C Chain: 6
Molecule details ›
Chain: 6
Length: 73 amino acids
Theoretical weight: 8.49 KDa
Source organism: Bos taurus
Cytochrome c oxidase subunit 7A1, mitochondrial Chain: 7
Molecule details ›
Chain: 7
Length: 59 amino acids
Theoretical weight: 6.68 KDa
Source organism: Bos taurus
Cytochrome c oxidase subunit 7B, mitochondrial Chain: 8
Molecule details ›
Chain: 8
Length: 56 amino acids
Theoretical weight: 6.37 KDa
Source organism: Bos taurus
Cytochrome c oxidase subunit 7C, mitochondrial Chain: 9
Molecule details ›
Chain: 9
Length: 47 amino acids
Theoretical weight: 5.45 KDa
Source organism: Bos taurus
Cytochrome c oxidase subunit 8B, mitochondrial Chain: s
Molecule details ›
Chain: s
Length: 46 amino acids
Theoretical weight: 4.97 KDa
Source organism: Bos taurus
Cytochrome c oxidase subunit 1 Chain: y
Molecule details ›
Chain: y
Length: 514 amino acids
Theoretical weight: 57.07 KDa
Source organism: Bos taurus
Cytochrome c oxidase subunit 2 Chain: z
Molecule details ›
Chain: z
Length: 227 amino acids
Theoretical weight: 26.04 KDa
Source organism: Bos taurus
NADH dehydrogenase [ubiquinone] 1 unknown subunit fragment Chains: Ab, Ah
Molecule details ›
Chains: Ab, Ah
Length: 134 amino acids
Theoretical weight: 11.42 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 Chain: Ac
Molecule details ›
Chain: Ac
Length: 70 amino acids
Theoretical weight: 8.09 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 unknown subunit fragment Chains: Ad, Ao, Ap, Av
Molecule details ›
Chains: Ad, Ao, Ap, Av
Length: 43 amino acids
Theoretical weight: 3.68 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 Chain: Ae
Molecule details ›
Chain: Ae
Length: 116 amino acids
Theoretical weight: 13.32 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 Chain: Af
Molecule details ›
Chain: Af
Length: 128 amino acids
Theoretical weight: 14.95 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 Chain: Ag
Molecule details ›
Chain: Ag
Length: 141 amino acids
Theoretical weight: 14.69 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 Chain: Aj
Molecule details ›
Chain: Aj
Length: 176 amino acids
Theoretical weight: 20.94 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 Chain: Ak
Molecule details ›
Chain: Ak
Length: 178 amino acids
Theoretical weight: 21.73 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 subunit C2 Chain: Al
Molecule details ›
Chain: Al
Length: 122 amino acids
Theoretical weight: 14.33 KDa
Source organism: Sus scrofa
Protein MGARP N-terminal domain-containing protein Chain: Am
Molecule details ›
Chain: Am
Length: 76 amino acids
Theoretical weight: 8.63 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 Chain: An
Molecule details ›
Chain: An
Length: 172 amino acids
Theoretical weight: 20.06 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 unknown subunit fragment Chains: Aq, As, Aw
Molecule details ›
Chains: Aq, As, Aw
Length: 76 amino acids
Theoretical weight: 6.49 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 unknown subunit fragment Chain: Ar
Molecule details ›
Chain: Ar
Length: 34 amino acids
Theoretical weight: 2.91 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 unknown subunit fragment Chain: At
Molecule details ›
Chain: At
Length: 17 amino acids
Theoretical weight: 1.46 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 unknown subunit fragment Chain: Au
Molecule details ›
Chain: Au
Length: 13 amino acids
Theoretical weight: 1.12 KDa
Source organism: Sus scrofa

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 5.4Å
Relevant EMDB volumes: EMD-9534